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Secondary antibodies bind to a primary antibody to assist in detection, amplification, sorting, and purification of target antigens, and are used in various biochemical techniques/assays (e.g., ELISA, western blot (WB), immunohistochemistry).
This antibody was obtained from a rabbit immunized with purified, recombinant Human IgG1 Fc (Uniprot#: P01857-1; Glu99-Lys330,C103S). HEK293 used as expression system.
Rabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide.
Immunogen
Human IgG
Classification
Monoclonal
Primary or Secondary
Secondary
Clone
RM116
Test Specificity
This antibody reacts to the Fc region of all gamma heavy chains of human immunoglobulins, including γ1, γ2, γ3, and γ4. No cross reactivity with other human heavy chains, mouse IgG, rat IgG, or goat IgG.
Based on IEP, this antibody reacts with heavy gamma chains on mouse IgG and light chains on all mouse immunoglobulins. This antibody has been pre-adsorbed against human serum proteins.